Tomato Brown Rugose Fruit Virus（ToBRFV）Test Strip Instructions

Ⅰ. Composition:

1. The QTY of ToBRFV test strip: 50 pcs

2. Plant tissue grinding bag: 50 pcs

3. Sample extraction buffer: 50mL 1 bottle

Ⅱ. Operating Steps:

1. Sample Preparation:

1.1 Method 1:

(1) Collection of plant samples: Take a leaf (30-50mg) about half the size of a thumbnail from a tomato leaf with a hand or scissors, such as flowers and other viral-like symptoms, and put it into the grinding plastic bag provided.

(2) Grinding and homogenizing of plant samples: Add 2 tubes of sample extraction solution (about 1 ml) to the grinding bag containing leaves with the provided straw, rub the plastic bag with hands like washing clothes until the leaves are completely crushed, and mix in the sample extraction solution.

1.2 Method 2:

(1) Tomato leaves with obvious virus-like symptoms such as Mosaic were collected. After weighing, take about 30-50 mg of leaf tissue and place it in a mortar.

(2) Add 1 ml of the extract into the mortar and grind the homogenate with a pestle for 1 minute.

(3) The homogenate is transferred to a 1.5ml plastic centrifuge tube, centrifuged at 5000 RPM for 3 minutes, or left for 3 minutes, and the supernatant is the sample detection liquid.

2. Sample Test:

Take out the test strip from the aluminum foil bag, lay it flat face up as shown in the figure below, and use a straw to absorb the above prepared sample extraction drops 3-4 drops into the sample adding hole. 

3. Result Interpretation:

The results were observed within 3 to 10 minutes, as shown in Figure 1 below, and the color was positive at both line C (control line) and line T (detection line). As shown in Figure 2 below, color rendering only on line C and no color rendering on line T is negative. If neither line C nor line T is colored, or only line T is colored and line C is not colored, the test strip is invalid.

Ⅲ. Notes:

1. When testing plants, take the amount of diseased leaves can not be more, strictly control the sampling amount, half or a thumbnail size is enough. If too many leaves are taken, the leaf green in the leaves will affect the detection color, and the detection sensitivity of the test strip is very high, and there will be no false negative phenomenon caused by insufficient sample quantity.

2. The strips are stored in a dry-sealed aluminum foil bag. Use now and get now.

3. Test strips are used for ToBRFV detection.

4. Tomato leaves with obvious virus symptoms should be collected during sampling. Symptoms of ToBRFV infection in melon plants include chlorosis, narrowing of flowers and leaves, pedicel necrosis, brown wrinkled fruit, and fruit shedding.

5. Sample by hand, scissors, or blade. After each sample is taken, clean it with running water.

6. The colloidal gold test strip must be used with the sample extraction solution provided, and no other sample extraction solution can be used.

7. Each straw can only be used for drawing and adding 1 sample. It is prohibited to use the same straw for drawing and adding samples of multiple samples.

Ⅳ. Storage Condition:

Store at room temperature from 4℃ to 35℃ away from light. Do not refrigerate or freeze.

Ⅴ. Production Date and Expiration Date:

Production dates are detailed in the box. It is valid for 12 months.